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bsmbi digested crispr interference crispri vector  (Addgene inc)


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    Structured Review

    Addgene inc bsmbi digested crispr interference crispri vector
    Bsmbi Digested Crispr Interference Crispri Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsmbi+digested+crispr+interference+crispri+vector/pCC_12+-+hU6-BsmBI-sgRNA(E%2BF)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE+(Plasmid+%23139097)/pmc11447040__125_2024_6214_MOESM1_ESM-121-32-37
    Average 92 stars, based on 4 article reviews
    bsmbi digested crispr interference crispri vector - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Sequencing:

    Article Title: Single-cell transcriptomic profiling of human pancreatic islets reveals genes responsive to glucose exposure over 24 h
    Article Snippet: .. We synthesised gRNAs targeting candidate genes and two non-targeting gRNAs (that do not recognize any sequence in the human genome [35]), as top- and bottom-strand oligos (IDT) and cloned them into the BsmBI-digested CRISPR interference (CRISPRi) vector (Addgene, #139097) according to the previously described instructions [36]. .. To produce the lentivirus expressing each CRISPRi system (dCas9-KRAB + gRNA), we transfected HEK293T cells with lentivirus packaging plasmids pMD2.G and psPAX2 (Addgene, #12259 and #12260) along with either CRISPRi plasmid targeting each candidate gene or the non-targeting control plasmids.

    Clone Assay:

    Article Title: Single-cell transcriptomic profiling of human pancreatic islets reveals genes responsive to glucose exposure over 24 h
    Article Snippet: .. We synthesised gRNAs targeting candidate genes and two non-targeting gRNAs (that do not recognize any sequence in the human genome [35]), as top- and bottom-strand oligos (IDT) and cloned them into the BsmBI-digested CRISPR interference (CRISPRi) vector (Addgene, #139097) according to the previously described instructions [36]. .. To produce the lentivirus expressing each CRISPRi system (dCas9-KRAB + gRNA), we transfected HEK293T cells with lentivirus packaging plasmids pMD2.G and psPAX2 (Addgene, #12259 and #12260) along with either CRISPRi plasmid targeting each candidate gene or the non-targeting control plasmids.

    CRISPR:

    Article Title: Single-cell transcriptomic profiling of human pancreatic islets reveals genes responsive to glucose exposure over 24 h
    Article Snippet: .. We synthesised gRNAs targeting candidate genes and two non-targeting gRNAs (that do not recognize any sequence in the human genome [35]), as top- and bottom-strand oligos (IDT) and cloned them into the BsmBI-digested CRISPR interference (CRISPRi) vector (Addgene, #139097) according to the previously described instructions [36]. .. To produce the lentivirus expressing each CRISPRi system (dCas9-KRAB + gRNA), we transfected HEK293T cells with lentivirus packaging plasmids pMD2.G and psPAX2 (Addgene, #12259 and #12260) along with either CRISPRi plasmid targeting each candidate gene or the non-targeting control plasmids.



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    bsmbi digested crispr interference crispri vector  (Addgene inc)
    92
    Addgene inc bsmbi digested crispr interference crispri vector
    Bsmbi Digested Crispr Interference Crispri Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsmbi+digested+crispr+interference+crispri+vector/pCC_12+-+hU6-BsmBI-sgRNA(E%2BF)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE+(Plasmid+%23139097)/pmc11447040__125_2024_6214_MOESM1_ESM-121-32-37
    Average 92 stars, based on 1 article reviews
    bsmbi digested crispr interference crispri vector - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

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